STAT6 Rabbit Monoclonal Antibody [Clone ID: UMAB300]

CAT#: UM900005

STAT6 Rabbit monoclonal antibody,clone UMAB300

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT6 (RC210065, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT6.(1:5000)

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  IHC staining of FFPE human solitary fibrous tumor tissue using anti-STAT6 rabbit monoclonal antibody (UM900005) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

Specifications

Product Data

• Clone Name: UMAB300
• Applications: IHC
• Recommended Dilution: IHC 1:1000,WB 1:5000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human STAT6 (NP_003144). The exact sequence is proprietary.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1.00mg/ml
• Purification: Purified from cell culture supernatant by affinity chromatography(protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 94.2 kDa
• Gene Name: signal transducer and activator of transcription 6
• Database Link:
  NP_003144
  Entrez Gene 6778 Human
  UniProt ID P42226
• Background: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
• Synonyms: D12S1644; IL-4-STAT; STAT6B; STAT6C

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway