Nestin (NES) Mouse Monoclonal Antibody [Clone ID: UMAB267]

CAT#: UM800157CF

Carrier-free (BSA/glycerol-free) NES mouse monoclonal antibody,clone UMAB267

Product Images

Immunohistochemical staining of paraffin-embedded Human glioma tissue using anti-NES mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800157) (1:200)

Specifications

Product Data

• Clone Name: UMAB267
• Applications: IHC
• Recommended Dilution: IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 99-349 of human NES (NP_006608) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nestin
• Database Link:
  NP_006608
  Entrez Gene 10763 Human
  UniProt ID P48681
• Background: This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
• Synonyms: Nbla00170

Reference Data

• Protein Families: ES Cell Differentiation/IPS