SP1 Rabbit Polyclonal Antibody

CAT#: TA899005

Rabbit Polyclonal SP1 Antibody

Product Images

Immunohistochemical staining of paraffin-embedded normal breast using anti-SP1 C/N TA899005 rabbit polyclonal antibody. HIER Citrate buffer pH6 ([B05-100B]) at 110C for 10 min, anti-SP1 diluted to 1:200. Detection was done with Polink1 Rabbit C/N D13-18 with DAB Kit. Image 40x magnification.

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  Immunohistochemical staining of paraffin-embedded normal placenta using anti-SP1 C/N TA899005 rabbit polyclonal antibody. HIER Citrate buffer pH6 ([B05-100B]) at 110C for 10 min, anti-SP1 diluted to 1:200. Detection was done with Polink1 Rabbit C/N D13-18 with DAB Kit. Image 40x magnification.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY SP1 (RC209154 , lane 2) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SP1 (TA899005, 1:1000).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB1:500, IHC 1:100 to 1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length protein
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Purification: Purified from the immunized serum by affinity chromatography
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86 kDa
• Gene Name: Sp1 transcription factor
• Database Link:
  Entrez Gene 6667 Human
  UniProt ID P08047
• Background: The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes; including cell differentiation; cell growth; apoptosis; immune responses; response to DNA damage; and chromatin remodeling. Post-translational modifications such as phosphorylation; acetylation; glycosylation; and proteolytic processing significantly affect the activity of this protein; which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.