p75 NGF Receptor (NGFR) Mouse Monoclonal Antibody [Clone ID: OTI8F1]

CAT#: TA812990S

NGFR mouse monoclonal antibody,clone OTI8F1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NGFR (Cat# RC207966, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NGFR (Cat# TA812990)(1:500).

• 

  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-NGFR monoclonal antibody (1:500).

• 

  Flow cytometric analysis of living 293T cells transfected with NGFR overexpression plasmid (RC207966), Red)/empty vector (PS100001, Blue) using anti-NGFR antibody (TA812990). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI8F1
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human Ngfr (NP_002498) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.18 kDa
• Gene Name: nerve growth factor receptor
• Database Link:
  NP_002498
  Entrez Gene 18053 MouseEntrez Gene 24596 RatEntrez Gene 4804 Human
  UniProt ID P08138
• Background: Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]
• Synonyms: CD271; Gp80-LNGFR; p75(NTR); p75NTR; TNFRSF16

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Neurotrophin signaling pathway