PASD5 (NPAS1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4C10]

CAT#: TA809593BM

NPAS1 mouse monoclonal antibody,clone OTI4C10, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NPAS1 (Cat# RC207926, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPAS1 (Cat# TA809593)(1:2000). Positive lysates LY419279 (100ug) and LC419279 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human skin tissue within the normal limits using anti-NPAS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809593) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue within the normal limits using anti-NPAS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809593) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-NPAS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809593) (1:500)

Specifications

Product Data

• Clone Name: OTI4C10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 319-522 of human NPAS1 (NP_002508) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.5 kDa
• Gene Name: neuronal PAS domain protein 1
• Database Link:
  NP_002508
  Entrez Gene 4861 Human
  UniProt ID Q99742
• Background: The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. Studies of a related mouse gene suggest that it functions in neurons. The exact function of this gene is unclear, but it may play protective or modulatory roles during late embryogenesis and postnatal development. [provided by RefSeq, Jul 2008]
• Synonyms: bHLHe11; MOP5; PASD5

Reference Data

• Protein Families: Druggable Genome, Transcription Factors