BNP (NPPB) Mouse Monoclonal Antibody [Clone ID: OTI6F8]

CAT#: TA809024S

NPPB mouse monoclonal antibody, clone OTI6F8 (formerly 6F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPPB (RC206590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPPB (1:2000). Positive lysates LY400900 (100ug) and LC400900 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NPPB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809024) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NPPB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809024) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NPPB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809024) (1:150)

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  NPPB (BNP/NT-ProBNP) Luminex ELISA with 8A9 Capture (TA809041) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.

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  NPPB (BNP/NT-ProBNP) Luminex ELISA with 3H2 Capture (TA809065) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.

• NPPB (BNP/NT-ProBNP) Luminex ELISA with 17A6 Capture (TA809025) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.

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Specifications

Product Data

• Clone Name: OTI6F8
• Applications: IHC, LMNX, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 27-102 of human NPPB (NP_002512) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11.9 kDa
• Gene Name: natriuretic peptide B
• Database Link:
  NP_002512
  Entrez Gene 4879 Human
  UniProt ID P16860
• Background: This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Jul 2008]
• Synonyms: BNP; Iso-ANP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Stem cell - Pluripotency