NFS1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2H5]

CAT#: TA805491BM

NFS1 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NFS1 (Cat# RC209405, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFS1 (Cat# TA805491). Positive lysates LY412089 (100ug) and LC412089 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805491)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805491)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805491)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NFS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805491)

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Specifications

Product Data

• Clone Name: OTI2H5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-299 of human NFS1(NP_066923) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NFS1 cysteine desulfurase
• Database Link:
  NP_066923
  Entrez Gene 18041 MouseEntrez Gene 84594 RatEntrez Gene 9054 Human
  UniProt ID Q9Y697
• Synonyms: COXPD52; HUSSY-08; IscS; NIFS

Reference Data

• Protein Pathways: Thiamine metabolism