GLI2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1G1]

CAT#: TA804601AM

GLI2 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (RC217291, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2. Positive lysates LY417413 (100ug) and LC417413 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804601)

Specifications

Product Data

• Clone Name: OTI1G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 729-1123 of human GLI2 (NP_005261) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 167.6 kDa
• Gene Name: GLI family zinc finger 2
• Database Link:
  NP_005261
  Entrez Gene 14633 MouseEntrez Gene 2736 Human
  UniProt ID P10070
• Synonyms: CJS; HPE9; PHS2; THP1; THP2

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer