FLIP (CFLAR) Mouse Monoclonal Antibody [Clone ID: OTI2H8]

CAT#: TA804581

CFLAR mouse monoclonal antibody, clone OTI2H8 (formerly 2H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CFLAR (Cat# RC200608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CFLAR antibody (Cat# TA804581). Positive lysates LY418376 (100ug) and LC418376 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CFLAR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804581)

Specifications

Product Data

• Clone Name: OTI2H8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-272 of human CFLAR (NP_003870) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.2 kDa
• Gene Name: CASP8 and FADD like apoptosis regulator
• Database Link:
  NP_003870
  Entrez Gene 8837 Human
  UniProt ID O15519
• Synonyms: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; CLARP; FLAME; FLAME-1; FLAME1; FLIP

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis