TdT (DNTT) Rabbit Monoclonal Antibody [Clone ID: OTIR1E10]

CAT#: TA592788

TDT Rabbit monoclonal antibody,clone OTIR1E10

Product Images

IHC staining of FFPE human thymus tissue with in the normal limits using anti-TDT mouse monoclonal antibody (TA592788) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 9.0 (B06C-50) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human thymoma tissue using anti-TDT mouse monoclonal antibody (TA592788) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 9.0 (B06C-50) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

Specifications

Product Data

• Clone Name: OTIR1E10
• Applications: IHC
• Recommended Dilution: IHC 1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to the N-terminus of human TDT (NP_001017520) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1.00mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Predicted Protein Size: 58.4 kDa
• Gene Name: DNA nucleotidylexotransferase
• Database Link:
  NP_004079
  Entrez Gene 1791 Human
  UniProt ID P04053
• Background: This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
• Synonyms: TDT

Reference Data

• Protein Pathways: Hematopoietic cell lineage, Non-homologous end-joining