TdT (DNTT) (NM_004088) Human Tagged ORF Clone

CAT#: RC217586

DNTT (Myc-DDK-tagged)-Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1

Product Images

Circular map for RC217586

• 

  Western blot validation of overexpression lysate (Cat# LY418231) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217586 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TdT
• Synonyms: TDT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217586 representing NM_004088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCACCACGAGCGTCCCACTTGAGCCCTCGGAAGAAGAGACCCCGGCAGACGGGTGCCTTGATGG
  CCTCCTCTCCTCAAGACATCAAATTTCAAGATTTGGTCGTCTTCATTTTGGAGAAGAAAATGGGAACCAC
  CCGCAGAGCGTTCCTCATGGAGCTGGCCCGCAGGAAAGGGTTCAGGGTTGAAAATGAGCTCAGTGATTCT
  GTCACCCACATTGTAGCAGAGAACAACTCGGGTTCGGATGTTCTGGAGTGGCTTCAAGCACAGAAAGTAC
  AAGTCAGCTCACAACCAGAGCTCCTCGATGTCTCCTGGCTGATCGAATGCATAGGAGCAGGGAAACCGGT
  GGAAATGACAGGAAAACACCAGCTTGTTGTGAGAAGAGACTATTCAGATAGCACCAACCCAGGCCCCCCG
  AAGACTCCACCAATTGCTGTACAAAAGATCTCCCAGTATGCGTGTCAGAGAAGAACCACTTTAAACAACT
  GTAACCAGATATTCACGGATGCCTTTGATATACTGGCTGAAAACTGTGAGTTTAGAGAAAATGAAGACTC
  CTGTGTGACATTTATGAGAGCAGCTTCTGTATTGAAATCTCTGCCATTCACAATCATCAGTATGAAGGAC
  ACAGAAGGAATTCCCTGCCTGGGGTCCAAGGTGAAGGGTATCATAGAGGAGATTATTGAAGATGGAGAAA
  GTTCTGAAGTTAAAGCTGTGTTAAATGATGAACGATATCAATCCTTCAAACTCTTTACTTCTGTATTTGG
  AGTGGGGCTGAAGACTTCTGAGAAGTGGTTCAGGATGGGTTTCAGAACTCTGAGTAAAGTAAGGTCGGAC
  AAAAGCCTGAAATTTACACGAATGCAGAAAGCAGGATTTCTGTATTATGAAGACCTTGTCAGCTGTGTGA
  CCAGGGCAGAAGCAGAGGCCGTCAGTGTGCTGGTTAAAGAGGCTGTCTGGGCATTTCTTCCGGATGCTTT
  CGTCACCATGACAGGAGGGTTCCGGAGGGGTAAGAAGATGGGGCATGATGTAGATTTTTTAATTACCAGC
  CCAGGATCAACAGAGGATGAAGAGCAACTTTTACAGAAAGTGATGAACTTATGGGAAAAGAAGGGATTAC
  TTTTATATTATGACCTTGTGGAGTCAACATTTGAAAAGCTCAGGTTGCCTAGCAGGAAGGTTGATGCTTT
  GGATCATTTTCAAAAGTGCTTTCTGATTTTCAAATTGCCTCGTCAAAGAGTGGACAGTGACCAGTCCAGC
  TGGCAGGAAGGAAAGACCTGGAAGGCCATCCGTGTGGATTTAGTTCTGTGCCCCTACGAGCGTCGTGCCT
  TTGCCCTGTTGGGATGGACTGGCTCCCGGCAGTTTGAGAGAGACCTCCGGCGCTATGCCACACATGAGCG
  GAAGATGATTCTGGATAACCATGCTTTATATGACAAGACCAAGAGGATATTCCTCAAAGCAGAAAGTGAA
  GAAGAAATTTTTGCGCATCTGGGATTGGATTATATTGAACCGTGGGAAAGAAATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217586 representing NM_004088
  Red=Cloning site Green=Tags(s)
  
  MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDS
  VTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIGAGKPVEMTGKHQLVVRRDYSDSTNPGPP
  KTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKD
  TEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSD
  KSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITS
  PGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSS
  WQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESE
  EEIFAHLGLDYIEPWERNA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004088
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004088.2
• RefSeq Size: 2071 bp
• RefSeq ORF: 1530 bp
• Locus ID: 1791
• UniProt ID: P04053
• Cytogenetics: 10q24.1
• Protein Pathways: Hematopoietic cell lineage, Non-homologous end-joining
• MW: 58.4 kDa
• Gene Summary: This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]