Moesin (MSN) Rabbit Polyclonal Antibody

CAT#: TA590685

Rabbit Polyclonal Moesin Antibody

Product Images

ELISA: Moesin Antibody

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  Immunofluorescent staining of HeLa cells using anti-MSN rabbit polyclonal antibody (TA590685).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MSN (Cat# RC205674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSN(Cat# TA590685). Positive lysates LY419318 (100ug) and LC419318 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MSN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: WB 1:5000~20000, IF 1:500,ELISA 1:100-1:2000
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the C Terminus Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.97mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: moesin
• Database Link:
  NP_002435
  Entrez Gene 17698 MouseEntrez Gene 81521 RatEntrez Gene 491924 DogEntrez Gene 711712 MonkeyEntrez Gene 4478 Human
  UniProt ID P26038
• Background: Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).
• Synonyms: HEL70
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration, Regulation of actin cytoskeleton