GADD45A Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2B7]

CAT#: TA507380BM

GADD45A mouse monoclonal antibody, clone OTI2B7 (formerly 2B7), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (RC204005, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A. Positive lysates LY419659 (100ug) and LC419659 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2B7
• Applications: WB
• Recommended Dilution: WB 1:4000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GADD45A(NP_001915) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.2 kDa
• Gene Name: growth arrest and DNA damage inducible alpha
• Database Link:
  NP_001915
  Entrez Gene 13197 MouseEntrez Gene 25112 RatEntrez Gene 1647 Human
  UniProt ID P24522
• Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
• Synonyms: DDIT1; GADD45

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, MAPK signaling pathway, p53 signaling pathway