TXNDC5 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1D4]

CAT#: TA507257AM

TXNDC5 mouse monoclonal antibody, clone OTI1D4 (formerly 1D4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TXNDC5 ([RC208568], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TXNDC5.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TXNDC5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and TXNDC5-Knockout 293T cells (KO, Cat# LC811412) were separated by SDS-PAGE and immunoblotted with anti-TXNDC5 monoclonal antibody TA507257, (1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

Specifications

Product Data

• Clone Name: OTI1D4
• Applications: WB
• Recommended Dilution: WB 1:400~4000
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TXNDC5(NP_071368) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.5 kDa
• Gene Name: thioredoxin domain containing 5
• Database Link:
  NP_071368
  Entrez Gene 105245 MouseEntrez Gene 695213 MonkeyEntrez Gene 81567 Human
  UniProt ID Q8NBS9
• Background: This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream MUTED (muted homolog) gene. [provided by RefSeq, Dec 2010]
• Synonyms: ERP46, Hcc-2, UNQ364, EndoPDI, MGC3178; thioredoxin domain containing 5

Reference Data

• Protein Families: Druggable Genome