DEF6 Mouse Monoclonal Antibody [Clone ID: OTI3B2]

CAT#: TA505371S

DEF6 mouse monoclonal antibody, clone OTI3B2 (formerly 3B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF6 (RC217877, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF6. Positive lysates LY411815 (100ug) and LC411815 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DEF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505371)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DEF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505371)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DEF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505371)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DEF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505371)

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  Anti-DEF6 mouse monoclonal antibody (TA505371) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF6 (RC217877).

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Specifications

Product Data

• Clone Name: OTI3B2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DEF6(NP_071330) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.7 kDa
• Gene Name: DEF6 guanine nucleotide exchange factor
• Database Link:
  NP_071330
  Entrez Gene 23853 MouseEntrez Gene 309642 RatEntrez Gene 50619 Human
  UniProt ID Q9H4E7
• Background: DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]). [supplied by OMIM]
• Synonyms: IBP; SLAT; SWAP70L

Reference Data

• Protein Families: Druggable Genome