NANP Mouse Monoclonal Antibody [Clone ID: OTI4F5]

CAT#: TA504598

NANP mouse monoclonal antibody, clone OTI4F5 (formerly 4F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP. Positive lysates LY407388 (100ug) and LC407388 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NANP monoclonal antibody at 1:2000 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NANP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504598)

Specifications

Product Data

• Clone Name: OTI4F5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NANP(NP_689880) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.6 kDa
• Gene Name: N-acetylneuraminic acid phosphatase
• Database Link:
  NP_689880
  Entrez Gene 67311 MouseEntrez Gene 311530 RatEntrez Gene 140838 Human
  UniProt ID Q8TBE9
• Synonyms: C20orf147; dJ694B14.3; HDHD4

Reference Data

• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways