ATIC Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4A10]

CAT#: TA504570BM

ATIC mouse monoclonal antibody, clone OTI4A10 (formerly 4A10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATIC (RC203490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATIC. Positive lysates LY418255 (100ug) and LC418255 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ATIC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI4A10
• Applications: WB
• Recommended Dilution: WB 1:500~1000
• Reactivities: Human, Dog, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATIC(NP_004035) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64.4 kDa
• Gene Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
• Database Link:
  NP_004035
  Entrez Gene 81643 RatEntrez Gene 108147 MouseEntrez Gene 488513 DogEntrez Gene 471 Human
  UniProt ID P31939
• Background: This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq]
• Synonyms: AICAR; AICARFT; HEL-S-70p; IMPCHASE; PURH

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Purine metabolism