USP7 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3D3]

CAT#: TA504060AM

USP7 mouse monoclonal antibody, clone OTI3D3 (formerly 3D3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP7 (RC213986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP7. Positive lysates LY401171 (100ug) and LC401171 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-USP7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-USP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504060)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-USP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504060)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504060)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504060)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504060)

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Specifications

Product Data

• Clone Name: OTI3D3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human USP7(NP_003461) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 128.1 kDa
• Gene Name: ubiquitin specific peptidase 7
• Database Link:
  NP_003461
  Entrez Gene 252870 MouseEntrez Gene 360471 RatEntrez Gene 7874 Human
  UniProt ID Q93009
• Synonyms: HAUSP; TEF1

Reference Data

• Protein Families: Druggable Genome, Protease