PSMA4 Mouse Monoclonal Antibody [Clone ID: OTI1H10]

CAT#: TA504023

PSMA4 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA4 (RC202786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA4. Positive lysates LY419117 (100ug) and LC419117 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMA4 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504023)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504023)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504023)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504023)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504023)

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Specifications

Product Data

• Clone Name: OTI1H10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMA4(NP_002780) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.49 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.3 kDa
• Gene Name: proteasome 20S subunit alpha 4
• Database Link:
  NP_002780
  Entrez Gene 26441 MouseEntrez Gene 29671 RatEntrez Gene 100687556 DogEntrez Gene 710417 MonkeyEntrez Gene 5685 Human
  UniProt ID P25789
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: HC9; HsT17706; PSC9

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome