PGAM2 Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: TA503424

PGAM2 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Product Images

Anti-PGAM2 mouse monoclonal antibody (TA503424) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2 (RC200701).

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  Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody (TA503424).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2. Positive lysates LY424823 (100ug) and LC424823 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.

Specifications

Product Data

• Clone Name: OTI2F5
• Applications: IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:50~100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant of human PGAM2(NP_000281) produced in HEK293 cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.72 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.6 kDa
• Gene Name: phosphoglycerate mutase 2
• Database Link:
  NP_000281
  Entrez Gene 24959 RatEntrez Gene 56012 MouseEntrez Gene 475495 DogEntrez Gene 720615 MonkeyEntrez Gene 5224 Human
  UniProt ID P15259
• Background: Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq]
• Synonyms: GSD10; PGAM-M; PGAMM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways