DOK2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2A3]

CAT#: TA503302BM

DOK2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (RC207621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2. Positive lysates LY418321 (100ug) and LC418321 (20ug) can be purchased separately from OriGene.

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  Anti-DOK2 mouse monoclonal antibody (TA503302) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK2 (RC207621).

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  HEK293T cells transfected with either RC207621 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK2 antibody (TA503302), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2A3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DOK2(NP_003965) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.2 kDa
• Gene Name: docking protein 2
• Database Link:
  NP_003965
  Entrez Gene 9046 Human
  UniProt ID O60496
• Background: The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq]
• Synonyms: p56DOK; p56dok-2

Reference Data

• Protein Families: Druggable Genome