ANKRD53 Mouse Monoclonal Antibody [Clone ID: OTI1A7]

CAT#: TA502505

ANKRD53 mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (RC206701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53. Positive lysates LY410969 (100ug) and LC410969 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ANKRD53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502505)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ANKRD53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502505)

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  Anti-ANKRD53 mouse monoclonal antibody (TA502505) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53 (RC206701).

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  HEK293T cells transfected with either RC206701 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANKRD53 antibody (TA502505), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: ankyrin repeat domain 53
• Database Link:
  NP_079209
  Entrez Gene 79998 Human
  UniProt ID Q8N9V6
• Synonyms: FLJ12056; FLJ36160