EMG1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B8]

CAT#: TA501209AM

Anti-EMG1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EMG1 (Cat# RC208885, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EMG1(Cat# TA501209). Positive lysates LY401906 (100ug) and LC401906 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EMG1 monoclonal antibody.

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  Anti-EMG1 mouse monoclonal antibody (TA501209) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1 (RC208885 ).

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  Flow cytometric Analysis of Hela cells, using anti-EMG1 antibody (TA501209), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-EMG1 antibody (TA501209), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1B8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EMG1(NP_006322) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.5 kDa
• Gene Name: EMG1 N1-specific pseudouridine methyltransferase
• Database Link:
  NP_006322
  Entrez Gene 14791 MouseEntrez Gene 312706 RatEntrez Gene 477708 DogEntrez Gene 722122 MonkeyEntrez Gene 10436 Human
  UniProt ID Q92979
• Background: This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome. COMPLETENESS: complete on the 3' end.
• Synonyms: C2F; Grcc2f; NEP1