HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: OTI1F4]

CAT#: TA501149

Anti-ARNT (HIF1 beta) mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNT (RC216724, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNT. Positive lysates LY400636 (100ug) and LC400636 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501149, Dilution 1:50)

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  Anti-ARNT mouse monoclonal antibody (TA501149) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNT (RC216724).

Specifications

Product Data

• Clone Name: OTI1F4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARNT (NP_001659) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86.5 kDa
• Gene Name: aryl hydrocarbon receptor nuclear translocator
• Database Link:
  NP_001659
  Entrez Gene 11863 MouseEntrez Gene 25242 RatEntrez Gene 405 Human
  UniProt ID P27540
• Background: The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene.
• Synonyms: bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma