Tristetraprolin (ZFP36) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI8B5]

CAT#: TA500626BM

ZFP36 mouse monoclonal antibody, clone 8B5, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFP36 (RC202049, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFP36. Positive lysates LY401161 (100ug) and LC401161 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-ZFP36 monoclonal antibody at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500626)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500626)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500626)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500626)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500626)

• Anti-ZFP36 mouse monoclonal antibody (TA500626) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ZFP36 (RC202049).

• HEK293T cells transfected with either RC202049 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ZFP36 antibody (TA500626), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI8B5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZFP36 (NP_003398) produced in HEK293T cell.
• Formulation: PBS (pH 7.4) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.8 kDa
• Gene Name: ZFP36 ring finger protein
• Database Link:
  NP_003398
  Entrez Gene 7538 Human
  UniProt ID P26651
• Synonyms: G0S24; GOS24; NUP475; RNF162A; TIS11; TTP; zfp-36