Goat Polyclonal Antibody

CAT#: TA398275

Fab Biotin Antibody Texas Red™ Conjugated

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1:500 - 1:2,500
  IHC: 1:200 - 1:1,000
  ELISA: 1:2,000 - 1:10,000
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: Fab Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-papain or anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: Texas Red
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Expiration date is one (1) year from date of receipt.
• Background: Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.
• Synonyms: Goat Fab Anti-Biotin Texas Red™ Conjugated Antibody, Goat Fab Fragment Anti-Biotin Antibody Texas Red™ Conjugation
• Note: Fab Anti-Biotin Antibody Texas Red Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.