DsRed Chicken Polyclonal Antibody

CAT#: TA397565S

RFP Antibody

Product Images

Western blot of Rockland's Chicken Anti-RFP Antibody. Loaded 0.1 µg of RFP protein (p/n 000-001-379) on a 4-20% gel and transferred to nitrocellulose membrane. Chicken anti-RFP Antibody was added at 1.0 µg/mL at RT for 2 hours. IRDye800® goat anti-Chicken (p/n 603-132-126) was added at 1:20,000 at RT for 45 minutes.

• 

  Western blot of Rockland's Chicken Anti-RFP Antibody. Loaded 0.1 µg of RFP protein (p/n 000-001-379) on a 4-20% gel and transferred to nitrocellulose membrane. Chicken anti-RFP Antibody was added at 1.0 µg/mL at RT for 2 hours. IRDye800® goat anti-Chicken (p/n 603-132-126) was added at 1:20,000 at RT for 45 minutes.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB: 1:2,000 - 1:3,000
  ELISA: 1:10,000
• Reactivities: RFP, rRFP, Tomato
• Host: Chicken
• Clonality: Polyclonal
• Immunogen: Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
• Specificity: RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is three (3) months from date of receipt.
• Database Link:
  UniProt ID Q9U6Y8
• Background: Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.
• Synonyms: chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
• Note: Anti-RFP is designed to detect recombinant RFP. Anti-RFP antibody has been tested by ELISA and western blot to detect RFP. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.