AVD Rabbit Polyclonal Antibody

CAT#: TA397069S

Avidin Antibody Texas Red™ Conjugated

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: WB: >1:1,000
  IF: User Optimized
  FC: User Optimized
  FLISA: >1:2,000
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Avidin (Hen Egg White)
• Specificity: Anti-Avidin Antibody Texas Red™ Conjugated was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Avidin. No reaction was observed against Streptavidin.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: Texas Red
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is one (1) year from date of receipt.
• Database Link:
  UniProt ID P02701
• Background: Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive.
• Synonyms: rabbit anti-Avidin Antibody Texas Red™ Conjugation, Texas Red™ conjugated rabbit anti-Avidin antibody, Avidin Texas Red™, Anti-Avidin Texas Red™ Antibody, Egg White
• Note: Rabbit Anti-Avidin Antibody Texas Red Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.