CAT Rabbit Polyclonal Antibody

CAT#: TA396991S

Catalase Antibody Peroxidase Conjugated

Product Images

Western Blot of Rabbit Anti-Catalase antibody. Lane R: Reduced samples of purified Catalase. Lane NR: Non-reduced samples of purified Catalase. Load: ~1ug of protein per lane. Primary antibody: Catalase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight488™ rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 59.9 kDa, 55 kDa for Catalase. Other band(s): none.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB: 1:500- 1:2,500
  ELISA: 1:2,000 - 1:10,000
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Catalase [Bovine Liver]
• Specificity: Catalase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is one (1) year from date of receipt.
• Database Link:
  UniProt ID P00432
• Background: Catalase is found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species.
• Synonyms: rabbit anti-Catalase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody
• Note: Anti-Catalase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Catalase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.