DEX Rabbit Polyclonal Antibody

CAT#: TA396860

Dextranase Antibody

Product Images

Both the antiserum and IgG fractions of anti-Dextranase (Penicillium) are shown to detect under reducing conditions of SDS-PAGE the 41,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:2,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: WB: 1:500 - 1:5,000
  ELISA: 1:5,000 - 1:20,000
• Reactivities: Penicillium
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Dextranase [Penicillium species]
• Specificity: Anti-DEXTRANASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species]. Cross reactivity against Dextranase from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
• Concentration: 1.0 mg/ml - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Expiration date is one (1) year from date of receipt.
• Database Link:
  UniProt ID P48845
• Background: Dextranase has catalytic activity: endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.
• Synonyms: rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX
• Note: DEXTRANASE Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Dextranase [Penicillium species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.