CALCA Monoclonal Antibody [Clone ID: 4D12.D4.F3.E2.H7.E7]

CAT#: TA396810S

Procalcitonin (4D12.D4.F3.E2.H7.E7) Antibody

Product Images

Western Blot of Mouse Anti-Procalcitonin antibody. Lane 1: MW. Lane 2: Procalcitonin Protein. Load: 5 µg per lane. Primary antibody: Procalcitonin antibody at NEAT overnight at 4°C. Secondary antibody: HRP Mouse IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 13.9 kDa.

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  Western Blot of Mouse Anti-Procalcitonin antibody. Lane 1: MW. Lane 2: Procalcitonin Protein. Load: 5 µg per lane. Primary antibody: Procalcitonin antibody at NEAT overnight at 4°C. Secondary antibody: HRP Mouse IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 13.9 kDa.

Specifications

Product Data

• Clone Name: 4D12.D4.F3.E2.H7.E7
• Applications: ELISA, WB
• Recommended Dilution: WB: 1:1,000 - 1:2,000
  ELISA: User Optimized
• Reactivities: Human
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: Procalcitonin Antibody was produced in mice prepared by repeated immunizations with full-length recombinant human Procalcitonin protein.
• Specificity: Anti-Procalcitonin Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human Procalcitonin. Cross-reactivity with Procalcitonin from other sources has not been determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Expiration date is one (1) year from date of receipt.
• Database Link:
  UniProt ID P06881
• Background: Anti-Procalcitonin antibody detects human Procalcitonin. Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, a N terminal residue, calcitonin and katacalcin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock. This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: mouse anti-procalcitonin antibody, calcitonin isoform CT preproprotein, calcitonin-related polypeptide alpha, CT, KC, CGRP, CALC1, CGRP1, CGRP-I
• Note: Anti-Procalcitonin [4D12.D4] antibody was tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.