Goat Polyclonal Antibody

CAT#: TA396730

Protein A Antibody Peroxidase Conjugated

Product Images

Western Blot of Goat anti-Protein A Antibody. Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 µg per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1:1,000-1:5,000
  IHC: 1:500-1:2,500
  ELISA: 1:10,000 - 1:50,000
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Protein A [Staphylococcus aureus]
• Specificity: Anti-Protein A (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus]. Cross reactivity against Protein A from other sources is unknown.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
• Concentration: 10.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Expiration date is one (1) year from date of receipt.
• Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins. Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Protein A (GOAT) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.
• Synonyms: goat anti-Protein A Antibody, HRP Conjugated goat anti-Protein A Antibody, Protein A Peroxidase, Protein A HRP Antibody
• Note: This product has been assayed against 1.0 ug of Protein A [Staphylococcus aureus] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product.