Morc3 Rabbit Polyclonal Antibody

CAT#: TA396539S

Morc3 Antibody

Product Images

Western Blot of Rabbit Anti-Morc3 antibody. Lane 1: C-Flag murine embryonic stem cells. Lane 2: C-Flag murine embryonic stem cells doxycycline induced. Load: 35 µg per lane. Primary antibody: mMorc3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 110 kDa for mouse Morc3. Other band(s): Sumoylated Morc runs higher.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB: 1:1000-1:5000
  ELISA: 1:10,000-1:50,000
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Morc3 whole rabbit serum was prepared by repeated immunizations with a mouse Morc3 recombinant protein.
• Specificity: Morc3 antibody was prepared from monospecific antiserum by delipidation and defibrination. The antibody is specific for mouse Morc3 in expressed cell lysates. Cross reactivity to other Morc proteins has not been determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 80 mg/mL - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is one (1) year from date of receipt.
• Gene Name: microrchidia 3
• Database Link:
  Entrez Gene 338467 Mouse
  UniProt ID Q6A0C2
• Background: The Morc (microrchidia) family of proteins are ATPases of the GHKL family. They have been implicated in transcriptional repression of genes and transposons, and higher order organization of DNA within the nucleus. Morc antibodies are ideal for researchers interested in Epigenetics, Cancer, and Cell cycle research.
• Synonyms: rabbit anti-Morc3 Antibody, MORC family CW-type zinc finger protein 3, Protein microrchidia, Morc antibody
• Note: Anti-Morc3 Antibody is tested for use in Western Blot and suitable for ChIP and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 110.8 kDa in size corresponding Morc3 by western blotting in the appropriate cell lysate or extract.