Eg5 (KIF11) Rabbit Polyclonal Antibody

CAT#: TA392934

Eg5 (phospho-T926) polyclonal antibody

Product Images

Western blot (WB) analysis of p-Eg5 (T926) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40µg) Lane2:HepG2 whole cell lysate(40µg) Lane3:CT-26 whole cell lysate(40µg) Lane4:C6 whole cell lysate(40µg) Lane5:LOVO whole cell lysate(40µg) Lane6:MCF-7 whole cell lysate(40µg)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Threonine 926
• Specificity: p-Eg5 (T926) pAb detects endogenous levels of Eg5 protein only when phosphorylated at Thr926.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 120 kDa
• Gene Name: kinesin family member 11
• Database Link:
  Entrez Gene 3832 Human
  UniProt ID P52732
• Background: Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules. Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development.
• Synonyms: EG5; KIF11; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; KNSL1; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; TRIP5
• Note: For research use only, not for use in diagnostic procedure.