Phospholipase C gamma 1 (PLCG1) Rabbit Polyclonal Antibody

CAT#: TA392894

PLC γ1 (phospho-Y783) polyclonal antibody

Product Images

Western blot (WB) analysis of p-PLC γ1 (Y783) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate(40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug) Lane4:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug) Lane5:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:The Kidney tissue lysate of Mouse(40ug)

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  Immunohistochemistry (IHC) analyzes of p-PLCG1 (Y783) pAb in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human PLC γ1 around the phosphorylation site of Tyrosine 783.
• Specificity: p-PLC γ1 (Y783) polyclonal antibody detects endogenous levels of PLC γ1 protein only when phosphorylated at Tyr783
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 148 kDa
• Gene Name: phospholipase C gamma 1
• Database Link:
  Entrez Gene 5335 Human
  UniProt ID P19174
• Background: Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actinregulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds α1β1 Integrin and modulates α1β1 Integrin-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells.
• Synonyms: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C2; PLC-148; PLC-gamma-1; PLC-II; PLC1; PLC2I; PLC148; PLCG1; PLCγ1
• Note: For research use only, not for use in diagnostic procedure.