PKC delta (PRKCD) Rabbit Polyclonal Antibody

CAT#: TA392654

PKC δ (phospho-T505) polyclonal antibody

Product Images

Western blot (WB) analysis of PKC δ (phospho-T505) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate Lane4:BV2 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB:1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human PKC δ around the phosphorylation site of Threonine 505.
• Specificity: p-PKC δ (T505) polyclonal antibody detects endogenous levels of PKC δ protein only when phosphorylated at Thr505.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 80 kDa
• Gene Name: protein kinase C delta
• Database Link:
  Entrez Gene 5580 Human
  UniProt ID Q05655
• Background: Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.
• Synonyms: nPKC-delta; PKCδ; PRKCD; Protein kinase C delta type; SDK1; Sphingosine-dependent protein kinase-1; Tyrosine-protein kinase PRKCD
• Note: For research use only, not for use in diagnostic procedure.