PKC alpha (PRKCA) Rabbit Polyclonal Antibody

CAT#: TA392653

PKC α (phospho-T638) polyclonal antibody

Product Images

Western blot (WB) analysis of PKC α (phospho-T638) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate Lane4:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane5:MCF-7 whole cell lysate

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  Immunohistochemistry (IHC) analyzes of p-PKC α (T638) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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  Immunoprecipitation of L02 cell lysate using PKC α (phospho-T638) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4) .Lane 1 is 30% input. The western blot was probed using SHP-2 (E574) pAb #TA392653.“↑”（supernatant）; “↓”（deposition）

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  Immunoprecipitation of L02 cell lysate using PKC α (phospho-T638) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using SHP-2 (E574) pAb #TA392653.“↑”（supernatant）; “↓”（deposition）

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human PKC α around the phosphorylation site of Threonine 638.
• Specificity: PKC α (phospho-T638) polyclonal antibody detects endogenous levels of PKC α protein when phosphorylated at Thr638.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 80 kDa
• Gene Name: protein kinase C alpha
• Database Link:
  Entrez Gene 5578 Human
  UniProt ID P17252
• Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.
• Synonyms: PKC-A; PKC-alpha; PKCA; PRKACA; PRKCA; Protein kinase C alpha type
• Note: For research use only, not for use in diagnostic procedure.