PTEN Rabbit Polyclonal Antibody

CAT#: TA392576

PTEN (Ab-370) polyclonal antibody

Product Images

Western blot (WB) analysis of PTEN (Ab-370) polyclonal antibody at 1:1000 dilution Lane1:THP-1 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug)

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  Immunofluorescence analysis of MCF-7 cells using PTEN antibody at dilution of 1:50.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500~1:1000 IF: 1:50~1:200
• Reactivities: Human, Rat, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to Human PTEN.
• Specificity: PTEN (Ab-370) polyclonal antibody detects endogenous levels of PTEN protein.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 50 kDa
• Gene Name: phosphatase and tensin homolog
• Database Link:
  Entrez Gene 5728 Human
  UniProt ID P60484
• Background: PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers. PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase. The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K). PTEN is a major negative regulator of the PI3K/Akt signaling pathway. PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity. PTEN regulates p53 protein levels and activity and is involved in G protein-coupled signaling during chemotaxis.
• Synonyms: Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
• Note: For research use only, not for use in diagnostic procedure.