RSK1 p90 (RPS6KA1) Rabbit Polyclonal Antibody

CAT#: TA392542

Rsk-1 (phospho-T359/S363) polyclonal antibody

Product Images

Western blot (WB) analysis of p-Rsk-1 (T359/S363) pAb at 1:2000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug)

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  Immunohistochemistry of paraffin-embedded Rat Brain using Rsk-1 (phospho-T359/S363) antibody at dilution of 1:50.

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  Immunofluorescence analysis of CT26 cells using Rsk-1 (phospho-T359/S363) antibody at dilution of 1:50.

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  Immunoprecipitation of HepG2 cell lysate using p-Rsk-1 (T359/S363) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using p-Rsk-1 (T359/S363) #TA392542. “↑”（supernatant）; “↓(deposition）

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Rsk-1 around the phosphorylation site of Thr359/Ser363.
• Specificity: Rsk-1 (phospho-T359/S363) polyclonal antibody detects endogenous levels of Rsk-1 protein only when phosphorylated at Thr359/Ser363.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 90 kDa
• Gene Name: ribosomal protein S6 kinase A1
• Database Link:
  Entrez Gene 6195 Human
  UniProt ID Q15418
• Background: The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development.
• Synonyms: 90 kDa ribosomal protein S6 kinase 1; MAPK-activated protein kinase 1a; MAPKAPK-1a; MAPKAPK1A; MAPKAP kinase 1a; MAP kinase-activated protein kinase 1a; p90-RSK 1; p90RSK1; p90S6K; Ribosomal protein S6 kinase alpha-1; Ribosomal S6 kinase 1; RPS6KA1; RSK-1; RSK1; S6K-alpha-1; S6K-α1; S6K α1; S6Kα1
• Note: For research use only, not for use in diagnostic procedure.