Caspase 9 (CASP9) Rabbit Polyclonal Antibody

CAT#: TA392498

Caspase-9 (T125) polyclonal antibody

Product Images

Western blot (WB) analysis of Caspase-9 (T125) polyclonal antibody at 1:1000 dilution Lane1:Jurkat whole cell lysate(40ug) Lane2:Jurkat treated with etoposide(25μM,5 hours) whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug)

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  Western blot (WB) analysis of Caspase-9 (T125) polyclonal antibody at 1:5000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Jurkat whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug) Lane4:Jurkat treated with etoposide(25μM,5 hours) whole cell lysate(40ug) Lane5:Jurkat whole cell lysate(40ug)

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  Immunoprecipitation of K562 cell lysates using Caspase-9 (T125) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using Caspase-9 (T125) pAb #TA392498.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000~1:2000 IP: 1:50~1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to Human Caspase-9.
• Specificity: Caspase-9 (T125) polyclonal antibody detects endogenous levels of Caspase-9 protein.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 37, 40/40, 46 kDa
• Gene Name: caspase 9
• Database Link:
  Entrez Gene 842 Human
  UniProt ID P55211
• Background: Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.
• Synonyms: APAF-3; Apoptotic protease-activating factor 3; Apoptotic protease Mch-6; CASP-9; Caspase-9 subunit p10; Caspase-9 subunit p35; ICE-LAP6; ICE-like apoptotic protease 6
• Note: For research use only, not for use in diagnostic procedure.