PLCG1 Mouse Antibody [Clone ID: M156]
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Specifications
Product Data | |
Clone Name | M156 |
Applications | ICC, IP, WB |
Recommended Dilution | WB: 1:1000 ICC: 1:100 |
Reactivities | Human, Rat, Mouse, Chicken |
Host | Mouse |
Isotype | IgG1 |
Immunogen | Clone (M156) was generated from a synthetic peptide corresponding to amino acids in the N-terminal region of human PLCγ1. This sequence is highly conserved in rat and mouse PLCγ1, and has low homology to PLCγ2. |
Specificity | The antibody detects a 150 kDa* protein in human A431, Hct116, and Jurkat cells, as well as in mouse brain. |
Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Concentration | lot specific |
Purification | Protein A Purified |
Conjugation | Unconjugated |
Storage | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Stability | After date of receipt, stable for at least 1 year at -20°C. |
Predicted Protein Size | 150 |
Database Link | |
Background | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulates the activity of PLC. PLCδ is activated by both receptor and nonreceptor tyrosine kinases. PLCγ1 forms a complex with EGF and PDGF receptors, which leads to phosphorylation at tyrosine 771, 783, and 1245. In addition, antigen receptor-induced activation of PLCγ1 leads to phosphorylation at both Tyr-775 and Tyr-783. These two sites are equally important for activation of enzymatic activity. |
Note | Protein G purified tissue culture supernatant. |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.