PRKCD Mouse Antibody [Clone ID: M242]

CAT#: TA389193

Anti-PKCδ (N-terminal region) Antibody

Specifications

Product Data

• Clone Name: M242
• Applications: ICC, WB
• Recommended Dilution: WB: 1:250
  ICC: 1:100
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG2b
• Immunogen: Clone (M242) was generated from a recombinant human protein that included amino acids residues in the N-terminal region. This sequence is conserved in rat and mouse PKCδ, and has low homology to other PKC family members.
• Specificity: This antibody detects a 78 kDa* protein corresponding to the molecular mass of PKCδ on SDS-PAGE immunoblots of adult mouse brain tissue lysate.
• Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
• Concentration: lot specific
• Purification: Protein A Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 78
• Database Link:
  UniProt ID Q05655
• Background: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity.
• Note: Protein G purified tissue culture supernatant.