CDC42 Mouse Antibody [Clone ID: M152]

CAT#: TA389099

Anti-Cdc42 Antibody

Specifications

Product Data

• Clone Name: M152
• Applications: ICC, WB
• Recommended Dilution: WB: 1:500
  ICC: 1:50
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Immunogen: Clone (M152) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.
• Specificity: The antibody detects a 21 kDa* protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.
• Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
• Concentration: lot specific
• Purification: Protein A Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 21
• Database Link:
  UniProt ID P60953
• Background: Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.
• Note: Protein G purified tissue culture supernatant.