Cxcl12 Rabbit Polyclonal Antibody

CAT#: TA388950

SDF-1 alpha (CXCL12) Antibody (biotin)

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Rat SDF-1 alpha by sandwich ELISA (using 100 ul/well), a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat SDF-1 alpha as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat SDF-1 alpha.
  Western Blot
  To detect Rat SDF-1 alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant Rat SDF-1 alpha is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat SDF-1 alpha. Anti-Rat SDF-1 alpha-specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: lot specific
• Purification: SDF-1 alpha-specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Background: SDF-1 alpha andbeta are stromal-derived, CXC chemokines that signal through the CXCR4 receptor. SDF-1alpha andbeta chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV-suppressive activity in cells expressing the CXCR4 receptor. Human and murine SDF-1 proteins act across species. SDF-1 alpha andbeta contain the four highly conserved cysteine residues present in CXC chemokines. The mature SDF-1alpha protein is the result of alternative splicing of the SDF-1 gene and contains 68 amino acid residues. Recombinant Rat SDF-1 alpha is a 7.9 kDa protein containing 68 amino acid residues.