Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommended Dilution | Sandwich ELISA: To detect Humanbeta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Humanbeta-NGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Humanbeta-NGF. Western Blot To detect Humanbeta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Humanbeta-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivities | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure recombinant Human beta-NGF. Anti-Human beta-NGF specific antibody was purified by affinity chromatography employing immobilized Human beta-NGF matrix. |
Concentration | lot specific |
Purification | beta-NGF specific antibody was purified by affinity chromatography employing immobilized Human beta-NGF matrix |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
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