Il6ra Rabbit Monoclonal Antibody [Clone ID: D7715A7]

CAT#: TA386280

Recombinant Anti-IL-6R (Clone D7715A7)

Product Images

Flow-cytometry using the Anti-IL-6R antibody D7715A7 (TA386280). Mouse splenocytes were stained with anti-Fluorescein IgG antibody (clone 4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (TA386280, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: D7715A7
• Applications: Bl, FC
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising Lewis rats with OKT4 cells.
• Specificity: This antibody is specific for murine IL-6R.
  
  This antibody binds to IL-6R-positive cells, and blocks IL-6 binding (Vink et al, 1990; Coulie et al, 1990). This antibody is able to inhibit the growth of IL6-dependent plasmacytoma T1033C2 in vitro (Coulie et al, 1990). Administration of this antibody to mice prior to challenge with T1033C2 resulted in a protection rate of 60% and prolonged survival in tumour-bearing mice (Vink et al, 1990). This antibody has been used in flow cytometry to assess IL-6Ra expression (Hoge et al, 2013). When added to dendritic cells subject to CD40 activation in vitro, this antibody neutralises IL-6 and ablates the effect of CD40 activation (Grohmann et al, 2001).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: interleukin 6 receptor, alpha
• Database Link:
  Entrez Gene 16194 Mouse
  UniProt ID P22272
• Synonyms: CD126; IL-6R; Il6r
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.