Vsir Rabbit Monoclonal Antibody [Clone ID: MH5A]

CAT#: TA386191

Recombinant Anti-PD-1H (Clone MH5A)

Specifications

Product Data

• Clone Name: MH5A
• Applications: Bl, ELISA, IHC
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: The antibody was generated by immunising Armenian hamsters with mPD-1HIg.
• Specificity: This antibody specifically binds PD-1H.
  
  The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-μg dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: V-set immunoregulatory receptor
• Database Link:
  Entrez Gene 74048 Mouse
  UniProt ID Q9D659
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.