Cd8a Rabbit Monoclonal Antibody [Clone ID: YTS 105.18]

CAT#: TA385775

Recombinant Anti-CD8 alpha (Clone YTS 105.18)

Product Images

Flow-cytometry using anti-CD8a antibody YTS 105.18 (TA385775) Mouse lymphocytes were stained with an isotype control (TA385644, panel A) or the rabbit-chimeric version of YTS 105.18 (TA385775, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: YTS 105.18
• Applications: Bl, FC, IF, IHC
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Ly-2-transfected mouse L cells.
• Specificity: Recognizes a non polymorphic epitope on the mouse CD8 alpha chain. This antibody has been reported to block MHC I dependent T cell responses in vitro and in vivo.
  
  This antibody can be used to stain for murine CD8 and can be used to block interaction with CD8.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD8 antigen, alpha chain
• Database Link:
  Entrez Gene 12525 Mouse
  UniProt ID P01731
• Synonyms: CD8; Leu2; MAL; OTTHUMP00000203721; p32
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.