SF2 (SRSF1) Rabbit Monoclonal Antibody [Clone ID: R07-9G7]

CAT#: TA385321

SF2 Rabbit monoclonal Antibody

Product Images

Western blot analysis of SF2 in 3T3 lysates using SF2 antibody.

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  Western blot analysis of SF2 in K562, C6 lysates using SF2 antibody

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  Immunohistochemistry analysis of paraffin-embedded Human tonsil using SF2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunocytochemistry analysis of SF2 (green) in 293T using SF2 antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R07-9G7
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000
  IHC: 1/50
  ICC/IF: 1/20-1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human SF2
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 28 kDa; Observed MW: 28 kDa
• Gene Name: serine/arginine-rich splicing factor 1
• Database Link:
  Entrez Gene 6426 Human
  UniProt ID Q07955
• Background: Swiss-Prot Acc.Q07955.Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
• Synonyms: 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; Asf; AW491331; Sf2; Sfrs1