NF-kB p65 (RELA) Rabbit Monoclonal Antibody [Clone ID: R09-3H2]

CAT#: TA385160

NF-κB p65 Rabbit monoclonal Antibody

Product Images

Western blot analysis of NF-KB p65 in Hela lysates using NF-KB p65 antibody.

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  Western blot detection of NF-kB p65 in Hela cell lysates using NF-kB p65 Rabbit mAb(1:1000 diluted).Predicted band size:60KDa.Observed band size:65KDa.

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  Immunohistochemistry of NF-kB p65 in paraffin-embedded Human breast cancer tissue using NF-kB p65 Rabbit mAb at dilution 1/2

Specifications

Product Data

• Clone Name: R09-3H2
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20
  ICC/IF: 1/50
  IP: 1/50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human NF-kB p65
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 60 kDa; Observed MW: 65 kDa
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  Entrez Gene 5970 Human
  UniProt ID Q04206
• Background: Swiss-Prot Acc.Q04206.NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression.
• Synonyms: MGC131774; NFKB3; p65